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User Guide	Variability Methods	Input	Output

This server calculates the sequence variability within a multiple sequence alignment using several variability metrics. Subsequently, the server can perform several tasks, such as masking the variability in the reference sequence, returning conserved fragments or mapping the sequence variability onto a provided 3D-structure.

INPUT Choose your input data type: Protein Alignment ( Valid formats are: CLUSTAL, FASTA, GCC/PILEUP) PDB File Paste your alignment. or Upload Alignment Get PDB file from PDB code    or Upload your PDB File     Chain ID

SEQUENCE VARIABILITY OPTIONS Select Variability Method Shannon Simpson Wu-Kabat Select Reference Sequence:  Consensus sequence  First sequence in alignment

OUTPUT TASKS Plot variability Plot variability Map structural variability     PDB file: Plot variability Map structural variability Map structural variability     PDB file: Map structural variability Mask sequence variability Return conserved fragment of length   6 7 9 9 10 12 15 20       Enter Variability threshold: