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 Tools    >> Genome Wide Prediction of Promiscous Epitope for Vaccine Design

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T-cell vaccines HLA-peptide binding HLA-coverage Proteosome Cleavage

Function

PEPVAC is a tool aimed to the development of fully covering multi-epitope vaccines against pathogenic organisms based on genome wide predictions of promiscous MHCI-restricted epitopes.

Description

T-cell epitopes are first anticipated based on their binding to HLA molecules using profile-matrices, and then filtered for immunoproteasomal cleavage using a probabilistic model. HLA molecules present many allelic variants with distinct peptides specificities, and thus peptide binding predictions are given for a selected set of HLA alleles covering a whole population including all major ethnities. These HLA alleles are grouped in sets (superantigenes) whose predicted binding peptides are largely overlapping. Only the peptides predicted to bind to all HLA alleles included in each superantigen set are selected as potential T-cell epitopes. Thus, indentification of these promiscous peptide binders allows to mininize the total number of predicted epitopes whithout compromising the population coverage required in the design of multi-epitope vaccines.

E-MAIL
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Enter your e-mail
GENOMES
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Select a genome
Upload genome (File with translated ORFs in FASTA format)
 

SUPERTYPES
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Select HLA-Supertype/s
A2:

A*0201, A*0202, A*0203, A*0205, A*0206, A*0207, A6802

A3:

A*0301, A*1101, A*3101, A*3301, A*6801, A*6601

A24:

A*2402, B*3801

B7:

B*0702, B*3501, B*5101, B*5102, B*5301, B*5401

B15:

A*0101, B*1501_B62, B1502

Population Coverage with selected supertypes [help]
PROTEASOME CLEAVAGE
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Filter: Model:
Citation:
  • Reche PA & Reinherz EL (2005) PEPVAC: a web server for multi-epitope vaccine development based on the prediction of supertypic MHC ligands. Nucleic Acids Res., 33:W138-42.
     
  • Reche PA, Reinherz EL (2007) Definition of MHC supertypes through clustering of MHC peptide-binding repertoires.. Methods Mol Biol., 409:163-173.
Credits: Pedro A. Reche
Hits since June/2004 Last updated:
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