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(Calculates DNA RNA/DNA melting temperature)

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1. Set the parameters for the run (or accept the defaults...)
input section
Select a set of sequences.

Use one of the following three fields:   (file must contain DNA sequences)

  1. To access a sequence from a database, enter the USA path here: (dbname:entry)

  2. Or, upload a sequence file from your local computer here:

  3. Or enter the sequence data manually here:

required section
Enter window size   (integer)    
Enter Shift Increment   (integer)    
Enter DNA concentration (nM)   (real number)    
Enter salt concentration (mM)   (real number)    
additional section
productsection section
unknown datatype 'toggle'
Enter percentage of formamide   (real number)    
Enter percent mismatch   (real number)    
Enter the product length   (integer)     (default is window size (20))
thermosection section
unknown datatype 'toggle'
Enter temperature   (real number)    
advanced section
Use RNA data values?  
output section
unknown datatype 'toggle'
Enter minimum temperature   (real number)    
Output graphic format  
Report format:
2. Submit to dan...
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